P26. Cancer associated fibroblasts contribute to the immune suppression of breast cancer by augmentation of the inflammatory products
نویسندگان
چکیده
Methods CAFs were isolated by explants culture of tumour tissue. The Fibroblast activated protein-alpha( FAP-a)-positive fibroblasts were co-cultured with splenocytes where the splenocyte proliferation and production of inflammatory and regulatory cytokines were assessed by ELISA. Also the inflammatory enzymes iNOS and the production of matrix metaloproteinases 2 and 9 by these cells were evaluated using Real-Time PCR.
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